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Objective@#To identify the functional connectivity characteristics of insula, sensory and social related brain regions in boys with autism spectrum disorder(ASD), to explore the central nervous basis of sensory abnormality affecting core symptoms in boys with ASD.@*Methods@#Resting state functional magnetic resonance imaging (rs fMRI) data were collected from 34 boys with ASD and 29 typical development boys (TD group). Based on functional connectivity analysis, the sensory related brain regions, insula, and social related brain regions were taken as regions of interest to calculate the functional connectivity (FC) level between the regions of interest, the differences between the two groups were compared and the results were corrected by FDR. The Autism Diagnostic Observation Schedule (ADOS), Childhood Autism Rating Scale (CARS) and Autism Spectrum Quotient-Children s Version (AQ-Child) were used to assess the core phenotypes of boys with ASD.@*Results@#Compared with the TD group, the levels of FC between tactile brain regions and insula, olfactory brain regions and insula, auditory brain regions and insula in boys with ASD group were significantly increased. The level of FC between the insula and bilateral amygdala，insula and the medial prefrontal cortex(mPFC) were significantly increased( P <0.05).Pearson correlation analysis showed that the level of FC between auditory brain region(BA42)and left insula in ASD group was negatively correlated with the scores of communication subscale of ADOS( r =-0.44)，social interaction subscale of ADOS( r =-0.43), communication & social interaction subscale of ADOS( r =-0.49),attention to details subscale of AQ-Child( r =-0.41). The level of FC between the right insula and right amygdala was positively correlated with the attention switch subscale of AQ-Child( r =0.38), the level of FC between right insula and mPFC was positively correlated with the scores of repetitive behavior subscale of ADOS( r =0.48), the attention switch subscale of AQ-Child( r =0.49), total scale subscale of AQ-Child( r =0.41), total scale of CARS( r =0.41)( P < 0.05 ).@*Conclusion@#The levels of FC between insula and sensory related, social related brain regions are abnormal in children with ASD, which have significant correlations with clinical symptoms. In depth studies can be conducted to explore underlying neutral mechanisms.
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Objective:To investigate the application and effects of WeChat official account in improving the nursing trainees' core post competency.Methods:The study included in 105 nursing trainees of Batch 2015 as experimental group, taking the nursing practice WeChat official account based on the routine clinical teaching pattern, and 95 nursing trainees of Batch 2014 as control group, taking traditional teaching mode. At the end of the internship, the scores of special examinations for nursing students and the national examination for nursing qualifications ("nursing qualification examination" for short) of the two group were compared, and the critical thinking ability, ability to acquire information lifelong learning ability, nurse-patient clinical communication ability and teaching satisfaction of the two groups of students were analyzed. SPSS 23.0 software was used for t test. Results:The scores of special examinations, nursing qualification examination and each module at main dimensions of the core post competency of nursing students in the experimental group were higher than those in the control group ( P<0.05). The nursing trainees in the experimental group were satisfied with the teaching effects of the WeChat official account. Conclusion:The WeChat official account in clinical nursing practice helps improve the quality of clinical practice teaching and assist in enhancing core post competency of nursing trainees.
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【Objective】 To investigate the role of thromboelastography (TEG) in the diagnosis and treatment of PICC related venous thrombosis in tumor patients, and provide basis for predicting PIC related venous thrombosis. 【Methods】 148 tumor patients who underwent PICC catheterization in our hospital from January to July 2019 were enrolled. The patients were subjected to TEG examination and ultrasound examination of limb blood vessels on catheterization side regularly one day before catheterization and 4 successive weeks after catheterization(once a week), respectively. The patients with venous thrombosis were subjected to TEG examination and vascular ultrasound regularly for 3 successive weeks(once a week) after anticoagulant therapy. The relationship between the occurrence and time of venous thrombosis and the changes of TEG parameters was analyzed. 【Results】 The pre-/post-catheterization TEG parameter of 81 patients without venous thrombosis indicated that coagulation state was normal (R value F=0.198, K value F=0.047, α value F=0.442, MAvalue F=0.079, CIvalue F=0.026, P>0.05). The TEG parameter of 67 patients, who developed asymptomatic venous thrombosis 1 to 3 weeks after catheterization(38 cases in 1 week, 16 in 2 weeks and 13 in 3 weeks), indicated the blood was hypercoagulable and the TEG parameter was statistically different from the pre-catheterization value(P0.05). 【Conclusion】 TEG can reflect the status of PICC related venous thrombosis in tumor patients.Strengthening TEG monitor in tumor patients 1 to 3 weeks after PICC catheterization is conducive to early detection, diagnosis and treatment for PICCrelated thrombosis, thus reducing the harm of venous thrombosis to patients.
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This paper summarized the operation cooperation and management of the first case of bilateral lung transplantation assisted with double extracorporeal membrane oxygenation (ECMO) for the recipient with novel coronavirus pneumonia (COVID-19) in the world. The bilateral lung transplantation assisted by double ECMO had been accomplished successfully on a recipient with COVID-19, who was supported by ventilator and ECMO, with other organs function basically normal. The operation took 405 min and went smoothly. The cooperation and management guidelines of the operation room included the following: setting up of COVID-19 emergency operation group quickly; listing and preparing of the detailed operation supplies; transferring of the whole system of lung transplantation operating room in Wuxi People's Hospital to the Wuxi No.5 People's Hospital (isolation hospital); reconstruction of a negative pressure operating room for lung transplantation; formulating and strictly implementing the guidelines and management process for the operation of patient with COVID-19.
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Objective@#To explore the effects of comprehensive temperature preserving nursing on the body temperature and the levels of lactic acid in lung transplantation patients.@*Methods@#Totally 46 patients underwent lung transplantation were randomly divided into experimental group and control group, with 23 patients in each group. Comprehensive insulation nursing measures were implemented in the experimental group, and conventional insulation measures were used in the control group. The rectal temperature and arterial blood lactic acid values of the two groups were recorded and compared before anesthesia (T0), after chest opening but before pulmonary artery occlusion (T1), after pulmonary artery occlusion (T2), during new lung transplantation (T3), after pulmonary artery opening (T4) and after surgery (T5).@*Results@#There was no statistically significant difference in rectal temperature and blood lactic acid between the two groups before anesthesia (t=1.53, 0.61, P>0.05). The rectal temperature of the experimental group were (36.55±0.14), (36.42±0.160), (36.33±0.13), (36.15±0.11), (36.45±0.10) ℃, which was higher than that of the control group (35.69±0.17, 35.52±0.19, 34.61±0.17, 34.47±0.13, 34.79±0.18) (F=3.24, P<0.01). The lactic acid level of the experimental group were (1.50±0.10), (2.97±0.15), (3.29±0.15), (2.15±0.10), (1.87±0.13) mmol/L, which was lower than that of the control group (2.37±0.10, 4.07±0.16, 5.15±0.21, 4.85±0.15, 4.72±0.17) (F=1.14, P<0.01). There was no statistically significant difference in rectal temperature between T0 and all operative stages in experimental group (F=0.39-1.41, P>0.05). The lactate levels of experimental group at T2 and T3 was higher than that before anesthesia (F=1.33, 1.16, P<0.01), and recovered to the pre-anesthesia level at T4 and T5 (F=0.59, 1.00, P>0.05).In the control group, the rectal temperature during each operation period was lower than that before anesthesia (F=1.17-2.51, P<0.01), and the lactic acid level was higher than that before anesthesia (F=0.51-2.25, P<0.01).@*Conclusion@#Comprehensive heat preservation nursing measures can maintain the relative stability of body temperature during lung transplantation, which is conducive to tissue perfusion and oxygenation.
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Objective To explore the effects of comprehensive temperature preserving nursing on the body temperature and the levels of lactic acid in lung transplantation patients. Methods Totally 46 patients underwent lung transplantation were randomly divided into experimental group and control group, with 23 patients in each group. Comprehensive insulation nursing measures were implemented in the experimental group, and conventional insulation measures were used in the control group. The rectal temperature and arterial blood lactic acid values of the two groups were recorded and compared before anesthesia (T0), after chest opening but before pulmonary artery occlusion (T1), after pulmonary artery occlusion (T2), during new lung transplantation (T3), after pulmonary artery opening (T4) and after surgery (T5). Results There was no statistically significant difference in rectal temperature and blood lactic acid between the two groups before anesthesia (t=1.53, 0.61, P>0.05). The rectal temperature of the experimental group were (36.55±0.14), (36.42±0.160), (36.33±0.13), (36.15±0.11), (36.45±0.10)℃, which was higher than that of the control group (35.69±0.17, 35.52±0.19, 34.61±0.17, 34.47±0.13, 34.79±0.18) (F=3.24, P<0.01). The lactic acid level of the experimental group were (1.50±0.10), (2.97±0.15), (3.29± 0.15), (2.15±0.10), (1.87±0.13) mmol/L, which was lower than that of the control group (2.37±0.10, 4.07± 0.16, 5.15±0.21, 4.85±0.15, 4.72±0.17) (F=1.14, P<0.01). There was no statistically significant difference in rectal temperature between T0 and all operative stages in experimental group (F=0.39-1.41, P>0.05). The lactate levels of experimental group at T2 and T3 was higher than that before anesthesia (F=1.33, 1.16, P<0.01), and recovered to the pre-anesthesia level at T4 and T5 (F=0.59, 1.00, P>0.05).In the control group, the rectal temperature during each operation period was lower than that before anesthesia (F=1.17-2.51, P<0.01), and the lactic acid level was higher than that before anesthesia (F=0.51-2.25, P<0.01). Conclusion Comprehensive heat preservation nursing measures can maintain the relative stability of body temperature during lung transplantation, which is conducive to tissue perfusion and oxygenation.
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This paper explores the reasonable construction of disease settlement standard system based on production process and source of costs, charging standards and the quantitative relationship between disease and settlement, putting forward clinical path minimum settlement standard and regulate a major guideline for settlement standard for local diseases by excluding the excessive service from patient's wills, high value consumables and high-priced drugs based on clinical path cost variation coefficient measured by disease typing and actual cost variation coefficient determined by class of hospital.
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Objective To explore the clinical effect of hysteroscopic eletrosurgery for abnormal uterine bleeding of endometrial polyps.Methods 126 patients with abnormal uterine bleeding of endometrial polyps were selected,and they were selected and randomly divided into control group and research group according to the digital table,63 cases in each group.The control group received traditional curettage surgery, the research group received hysteroscopic eletrosurgery.The intraoperative blood loss,operative time,length of hospital stay,postoperative menstruation recovery time,therapeutic effect and complications were compared between the two groups.Results The intraoperative blood loss and operation time of the research group were significantly lower than those of the control group (t1=7.44,P1<0.01,t2=7.78,P2<0.01).And there were no statistical differences between the two groups in length of hospital stay and postoperative menstruation recovery time(t1=1.82,P1=0.07,t2=1.71,P2=0.09).The total cure rate of the research group was 100.00%,which of the control group was 92.14%,the difference was statistically significant(x2=8.18,P=0.00).The incidence rate of complications of the research group was 3.17%, which of the control group was 17.50%,the difference was statistically significant(x2=11.08,P=0.00).Conclusion Hysteroscopic eletrosurgery for abnormal uterine bleeding of endometrial polyps has remarkable clinical effects and low incidence of complications,and it is worthy of clinical application.
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Objective To explore the in vitro antibacterial effect of tanreqing injection combined with cefuroxime sodium injection against staphylococcus aureus. Methods The MIC of tanreqing injection or cefuroxime sodium injection against staphylococcus aureus was detected by microamount dilution method.The antibacterial activity of tanreqing injection combined with cefuroxime sodium injection was determined by a chess board dilution method and assessed according to FIC index. Results The MIC of tanreqing injection and cefuroxime sodium injection against staphylococcus aureus was 1∶256 and 2 μg . mL-1 , respectively. While combined with each other, the MIC of tanreqing injection and cefuroxime sodium injection against staphylococcus aureus was 1∶4 096 and 0. 125 μg . mL-1 , respectively. The FIC index of tanreqing injection combined with cefuroxime sodium injection against staphylococcus aureus was 0. 125. Conclusion Tanreqing injection has a synergistic antibacterial effect against staphylococcus aureus when it was combined with cefuroxime sodium injection.
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Objective To summarize the experience of harvesting and using the lungs from donation after citizens death.Method From November 2007 to December 2013,19 cases of potential donation after brain death (DBD) and donation after cardiac death (DCD) were evaluated,including 9 cases of DCD and 10 cases of DBD.All the patients received the tests of sputum culture,bedside bronchoscopes,chest X rays,and blood gas analysis.After clear evaluation,3 cases of DCD and 3 cases of DBD were discharged from the group for bilateral inflammatory infiltration and poor oxygenation index,and the rest one case of DCD was precluded due to long warm ischemic time (＞60 min).The donor lungs from remaining 12 cases were harvested successfully after the declaration of brain death or cardiac death.The donors suitable for the transplant procedure were transported to our transplant center.Result Twelve lung transplants were performed successfully,including 10 cases of bilateral lung transplantation and 2 cases of right single lung transplantation.Two patients was complicated with severe infection and died of sepsis postoperatively,and the remaining 10 patients all recovered uneventfully with dramatic improvement of pulmonary function.During the follow-up period,all the patients lived an active life style with high quality of life.The mean survival time was 34.7 months (4-60 months).Conclusion Lung transplantation using DCD and DBD can be successfully performed after adequate preoperative evaluation of donor lung and abundant preparation for donor harvesting.
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Objective To investigate the curative effect and safety of endoscopic nose biliary drainage (ENBD) through endoscopic retrograde cholangiopancreatography (ERCP) to treat for elderly patients with acute severe cholangitis in primary hospital.Methods Forty-two elderly patients with acute severe cholangitis were collected,the patients received ENBD through ERCP.The changes of clinical symptoms and signs,imaging examinations and laboratory examinations before and after treatment were compared.Results All 42 patients received ENBD through ERCP,40 patients were cured,1 patient failed in ERCP and then was turned to surgical operation,and 1 patient whose cholangitis condition got better died of respiratory failure because of chronic obstructive pulmonary disease (COPD).Conclusion ENBD through ERCP is a safety and effective treatment which was used to treat for elderly patients with acute severe cholangitis in primary hospital,so this technique is worthy popularizing in primary hospital.
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We induced embryogenic calli (EC) and non-embryogenic calli (NEC) from flower filaments of Vitis vinifera L. cv. Chardonnay about 10 days before full bloom. The callus were sub-cultured, observed and verified by somatic embryo induction. PCR primers for VvSUC12 and VvSCU27 were designed according to the corresponding sequences in GenBank. After RNA extraction with RNAplant for EC and NEC cell lines, we synthesized the 1st strand DNA for semi quantitative RT-PCR, and normalized the density of the bands against house-keeping gene Actin. The results of 31 cycles semi-quantitative RT-PCR showed that VvSUC12 was highly expressed in both EC and NEC, with higher expression intensity in NEC than in EC, but not reached the significant level; while the expression of VvSUC27 was only detected in EC, and the expression level was significantly lower than that of VvSUC12. We increased the semi-quantitative RT-PCR cycle number to 35 and found that VvSUC27 gene was weakly expressed in NEC, in EC the intensity of the band was increased comparing with 31 cycles, and the expression level was higher than that of NEC. The paper discussed the differential expression of the two sucrose transporters and their relationship with the sucrose in the tissue culture medium.
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Culture Techniques , Methods , Gene Expression Regulation, Developmental , Gene Expression Regulation, Plant , Genes, Plant , Membrane Transport Proteins , Genetics , Plant Proteins , Genetics , Reverse Transcriptase Polymerase Chain Reaction , Vitis , Embryology , Genetics , MetabolismABSTRACT
OBJECTIVE To discuss the effect of the central sterilization and supply center in controlling the infection in hospital.METHODS Eliminated the old supply pattern,to implement centralized management for the supply office.The whole sterilization and supply center was rebuilded according to the standard,cleared the function,standardized the procedure and strengthened the management.RESULTS The centralized sterilization and supply center could form effective and standard circulatory system for cleaneiness,sterilization and antiseptic,and ensure the quality of disinfectants.CONCLUSIONS The centralized sterilization and supply center is better to the management and control of quality, it can reduce the pollution,simplify operational procedures,reduce hospital infection,and realize the zero flaw of aseptic goods.
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Objective To investigate the effect of Dihuang Guanshitong Oral Liquids on expression of E-cadherin of esophageal carcinoma rats. Methods: esophageal membranous epithelium were examined for E-Cadherin by Western blotting. Results: E-cadherin showed obvious low expression in the model group, compared with the normal group (P